A novel diterpene synthase from the sandfly Lutzomyia longipalpis produces the pheromone sobralene
Publication date
2024-02-28Creators
Ducker, Charles
Baines, Cameron
Guy, Jennifer
Santana, Antonio Euzebio Goulart
Pickett, John A.
Oldham, Neil J.
Metadata
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Raw mass spectrometry data in support of publication (submitted to PNAS). Abstract: The phlebotomine sandfly, Lutzomyia longipalpis, a major vector of the Leishmania parasite, uses terpene pheromones to attract conspecifics for mating. Examination of the L. longipalpis genome revealed a putative terpene synthase (TPS), which – upon heterologous expression in, and purification from, E. coli – yielded a functional enzyme. The TPS, termed LzTPS, converted geranyl diphosphate (GPP) into a mixture of monoterpenes with low efficiency, of which β-ocimene was the major product. (E,E)-farnesyl diphosphate (FPP) principally produced small amounts of (E)-β -farnesene, whilst (Z,E)- and (Z,Z)-FPP yielded a mixture of bisabolene isomers. None of these mono- and sesquiterpenes are known volatiles of L. longipalpis. Notably, however, when provided with (E,E,E)-geranylgeranyl diphosphate (GGPP), LzTPS gave sobralene as its major product. This diterpene pheromone is released by certain chemotypes of L. longipalpis, in particular those found in the Ceará state of Brazil. Minor diterpene-components were also seen as products of the enzyme that matched those seen in a sandfly pheromone extract.
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Subjects
- Terpenese
- Biosynthesis
- Pheromones
- Sandfly fever
- terpene biosynthesis, diterpene synthase, sex/aggregation pheromone, sandfly, sobralene
- Biological Sciences::Molecular biology, biophysics & biochemistry
- Q Science::QD Chemistry
- Q Science::QP Physiology
- R Medicine::RC Internal medicine
Divisions
- University of Nottingham, UK Campus::Faculty of Science::School of Chemistry
Deposit date
2024-01-30Data type
Digital mass spectrometry dataFunders
- Biotechnology & biological Sciences Research Council
Grant number
- BB/V003933/1
Data collection method
Data from Thermo GC-MS system, Thermo LC-MS system and Waters MSResource languages
- en_US