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dc.contributor.authorDucker, Charles
dc.contributor.authorBaines, Cameron
dc.contributor.authorGuy, Jennifer
dc.contributor.authorSantana, Antonio Euzebio Goulart
dc.contributor.authorPickett, John A.
dc.contributor.authorOldham, Neil J.
dc.date.accessioned2024-01-30T11:00:46Z
dc.date.available2024-01-30T11:00:46Z
dc.date.issued2024-02-28
dc.identifier.urihttps://rdmc.nottingham.ac.uk/handle/internal/11000
dc.description.abstractRaw mass spectrometry data in support of publication (submitted to PNAS). Abstract: The phlebotomine sandfly, Lutzomyia longipalpis, a major vector of the Leishmania parasite, uses terpene pheromones to attract conspecifics for mating. Examination of the L. longipalpis genome revealed a putative terpene synthase (TPS), which – upon heterologous expression in, and purification from, E. coli – yielded a functional enzyme. The TPS, termed LzTPS, converted geranyl diphosphate (GPP) into a mixture of monoterpenes with low efficiency, of which β-ocimene was the major product. (E,E)-farnesyl diphosphate (FPP) principally produced small amounts of (E)-β -farnesene, whilst (Z,E)- and (Z,Z)-FPP yielded a mixture of bisabolene isomers. None of these mono- and sesquiterpenes are known volatiles of L. longipalpis. Notably, however, when provided with (E,E,E)-geranylgeranyl diphosphate (GGPP), LzTPS gave sobralene as its major product. This diterpene pheromone is released by certain chemotypes of L. longipalpis, in particular those found in the Ceará state of Brazil. Minor diterpene-components were also seen as products of the enzyme that matched those seen in a sandfly pheromone extract.en_UK
dc.language.isoen_USen_UK
dc.publisherThe University of Nottinghamen_UK
dc.rightsCC-BY-NC-ND*
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.lcshTerpeneseen_UK
dc.subject.lcshBiosynthesisen_UK
dc.subject.lcshPheromonesen_UK
dc.subject.lcshSandfly feveren_UK
dc.titleA novel diterpene synthase from the sandfly Lutzomyia longipalpis produces the pheromone sobraleneen_UK
dc.identifier.doihttp://doi.org/10.17639/nott.7364
dc.subject.freeterpene biosynthesis, diterpene synthase, sex/aggregation pheromone, sandfly, sobraleneen_UK
dc.subject.jacsBiological Sciences::Molecular biology, biophysics & biochemistryen_UK
dc.subject.lcQ Science::QD Chemistryen_UK
dc.subject.lcQ Science::QP Physiologyen_UK
dc.subject.lcR Medicine::RC Internal medicineen_UK
uon.divisionUniversity of Nottingham, UK Campus::Faculty of Science::School of Chemistryen_UK
uon.funder.controlledBiotechnology & biological Sciences Research Councilen_UK
uon.datatypeDigital mass spectrometry dataen_UK
uon.grantBB/V003933/1en_UK
uon.collectionmethodData from Thermo GC-MS system, Thermo LC-MS system and Waters MSen_UK
uon.identifier.risproject4225901en_UK


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