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dc.contributor.authorHook, Andrew
dc.date.accessioned2023-03-09T13:25:40Z
dc.date.available2023-03-09T13:25:40Z
dc.date.issued2023-04-30
dc.identifier.urihttps://rdmc.nottingham.ac.uk/handle/internal/10455
dc.description.abstractPentosan polysulfate sodium (PPS) is a glycosaminoglycan mimetic that is the only current active pharmaceutical ingredient (API) for the oral treatment of interstitial cystitis. PPS is an inherently complex API due to variations in chain length, backbone structure, sulfation pattern and end group making discriminating between batch-to-batch as well as manufacturer-to-manufacturer differences difficult. In this study high throughput liquid handling and time-of-flight secondary ion mass spectrometry (ToF-SIMS) is used to enable the rapid chemical analysis (450 samples in 6 hours) of small amounts (≈150 ng) of PPS. Chemical discrimination between PPS produced by different manufacturers and, in one case, batch-to-batch variation of the same manufacturer is achieved. Analysis of samples using a 3D-OrbiSIMS instrument is used to provide confident assignment of the discriminating ions to enable structural interpretation of the variance observed between samples. Together with analysis of samples spiked with NaOAc or NaSO4, free sulphate and acetate along with sulphate pattern are implicated as key differences between manufacturers/batches. This approach is likely to be highly beneficial for the rapid quality control of PPS.en_UK
dc.language.isoenen_UK
dc.publisherThe University of Nottinghamen_UK
dc.rightsCC-BY*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.lcshPrincipal components analysisen_UK
dc.subject.lcshSecondary ion mass spectrometryen_UK
dc.subject.lcshMass spectrometersen_UK
dc.subject.lcshInterstitial cystitisen_UK
dc.titleSecondary ion mass spectrometry analysis of pentosan polysulfateen_UK
dc.identifier.doihttp://doi.org/10.17639/nott.7285
dc.subject.freeToF-SIMS; 3D-OrbiSIMS; Principal component analysisen_UK
dc.subject.jacsPhysical sciences::Chemistry::Medicinal chemistryen_UK
dc.subject.lcQ Science::QD Chemistryen_UK
uon.divisionUniversity of Nottingham, UK Campus::Faculty of Science::School of Pharmacyen_UK
uon.funder.controlledOtheren_UK
uon.datatypeSecondary ion mass spectrometry analysis of pentosan polysulfatesen_UK
uon.funder.freeUniversity of Nottinghamen_UK
uon.collectionmethodToF-SIMS and 3D-OrbiSIMSen_UK
uon.institutes-centresUniversity of Nottingham, UK Campusen_UK


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