Secondary ion mass spectrometry analysis of pentosan polysulfate
Description
Pentosan polysulfate sodium (PPS) is a glycosaminoglycan mimetic that is the only current active pharmaceutical ingredient (API) for the oral treatment of interstitial cystitis. PPS is an inherently complex API due to variations in chain length, backbone structure, sulfation pattern and end group making discriminating between batch-to-batch as well as manufacturer-to-manufacturer differences difficult. In this study high throughput liquid handling and time-of-flight secondary ion mass spectrometry (ToF-SIMS) is used to enable the rapid chemical analysis (450 samples in 6 hours) of small amounts (≈150 ng) of PPS. Chemical discrimination between PPS produced by different manufacturers and, in one case, batch-to-batch variation of the same manufacturer is achieved. Analysis of samples using a 3D-OrbiSIMS instrument is used to provide confident assignment of the discriminating ions to enable structural interpretation of the variance observed between samples. Together with analysis of samples spiked with NaOAc or NaSO4, free sulphate and acetate along with sulphate pattern are implicated as key differences between manufacturers/batches. This approach is likely to be highly beneficial for the rapid quality control of PPS.
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Subjects
- Principal components analysis
- Secondary ion mass spectrometry
- Mass spectrometers
- Interstitial cystitis
- ToF-SIMS; 3D-OrbiSIMS; Principal component analysis
- Physical sciences::Chemistry::Medicinal chemistry
- Q Science::QD Chemistry
Divisions
- University of Nottingham, UK Campus::Faculty of Science::School of Pharmacy
Research institutes and centres
- University of Nottingham, UK Campus
Deposit date
2023-03-09Data type
Secondary ion mass spectrometry analysis of pentosan polysulfatesFunders
- Other
- University of Nottingham
Data collection method
ToF-SIMS and 3D-OrbiSIMSResource languages
- en