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Badger Liver Elemental Concentrations

dc.contributor.authorSartorius, Andrea
dc.contributor.otherBennett, Malcolm
dc.contributor.otherCorbetta, Davide
dc.contributor.otherGrau Roma, Llorenç
dc.contributor.otherSandoval Barron, Elsa
dc.contributor.otherSwift, Ben
dc.coverage.spatialCheshire, Derbyshire, Nottinghamshire, Leicestershire, Warwickshire, and Northamptonshireen_UK
dc.date.accessioned2022-09-13T07:46:19Z
dc.date.available2022-09-13T07:46:19Z
dc.date.issued2022-09-13
dc.identifier.urihttps://rdmc.nottingham.ac.uk/handle/internal/9558
dc.description.abstractThis dataset contains the concentrations of 30 elements found in the livers of 448 badgers collected in the English Midlands in 2016 - 2017. All concentrations are in mg / kg dry weight.en_UK
dc.language.isoenen_UK
dc.publisherThe University of Nottinghamen_UK
dc.rightsCC-BY*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.lcshBadgersen_UK
dc.subject.lcshTrace elements in the bodyen_UK
dc.subject.lcshBadgers -- Heavy metal contenten_UK
dc.titleBadger Liver Elemental Concentrationsen_UK
dc.identifier.doihttp://doi.org/10.17639/nott.7237
dc.subject.freeEuropean Badgers, elemental concentrations, trace metals, heavy metals, biomonitoringen_UK
dc.subject.jacsVeterinary Sciences, Agriculture & related subjects::Animal science::Animal health::Animal physiologyen_UK
dc.subject.jacsVeterinary Sciences, Agriculture & related subjects::Animal science::Animal health::Animal pathologyen_UK
dc.subject.lcQ Science::QL Zoologyen_UK
dc.date.collection2016 - 2017en_UK
dc.coverage.coordinatesAll badgers were collected within -3.508178, 51.522730: 0.427985, 53.700807/en_UK
uon.divisionUniversity of Nottingham, UK Campus::Faculty of Medicine and Health Sciences::School of Veterinary Medicine & Scienceen_UK
uon.funder.controlledNatural Environment Research Councilen_UK
uon.datatypeRaw concentrationsen_UK
uon.funder.freeNatural Resources Walesen_UK
uon.funder.freeDepartment for Environment, Food and Rural Affairsen_UK
uon.grantNE/L002604/1en_UK
uon.grantSE3054 / OJEU 28406en_UK
uon.collectionmethodFound badger carcasses were collected in England across Cheshire, Derbyshire, Nottinghamshire, Leicestershire, Warwickshire, and Northamptonshire in 2016 – 2017, as part of a study on bovine tuberculosis in badgers (Figure 1; Swift et al., 2021). These badger carcasses were primarily collected on roadsides by citizen scientists. Liver samples, chosen because of their key role in element regulation and bioaccumulation (Kalisińska, 2019), were extracted from 448 of these badgers and freeze dried before being acid digested. Between 0.1 – 0.2 g of liver, along with 4 mL 70% HNO3 and 1 mL H2O2, were incubated on a hotplate block digester at 95 ˚C. After two hours, the samples were allowed to cool before being dispensed into plastic volumetric flasks, made up to 50 mL with MilliQ water (18.2 MΩ cm; Millipore Corporation, Darmstadt, Germany), and gently mixed. The samples were then left for at least ten minutes to allow for heavier material to settle to the bottom of the tube, and 10 mL of the supernatant solutions were decanted and stored at ambient temperature. The solutions were diluted 1-in-10 with MilliQ water prior to elemental analysis by ICP-MS. A certified reference material for trace elements in biological samples (BRC-185R Bovine Liver [trace elements]) was run for quality assurance purposes (recovery values for specific elements were: As [74.9%], Cd [103.9%], Cu [94.9%], Mn [99.1%], Pb [94.2%], Se [90.1%], and Zn [101.2%]). For each element, the operational limit of detection (LOD) was calculated as three times the standard deviation of the concentrations measured in 10 blank digestion samples run alongside the badger livers (Table 1). A value of 0.5*LOD was used in instances where the elemental concentration was lower than the LOD (Kushner, 1976). All concentrations were calculated as mg kg-1 dry weight; comparisons with published fresh weight concentrations were done assuming a liver water content of 72.1%, as described for European badgers by Kalisińska et al. (2009).en_UK
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