Aquatic invertebrate communities and Pb body burdens at abandoned Pb mine sites

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Publication date
2022-06-13
Creators
Sartorius, Andrea
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Description
This dataset contains the raw data for the aquatic invertebrate communities and Pb body burdens, as found during this study. The invertebrates were sampled from streams in abandoned Pb mine sites and in nearby control sites. The communities were identified to genus level (when possible), while the Pb body burdens were determined based on invertebrate families.
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Subjects
  • Aquatic invertebrates
  • Invertebrate communities
  • Lead
  • Water -- Pollution
  • Aquatic organisms -- Effect of water pollution on
  • aquatic invertebrates; communities; metals; pollution; contamination; lead; Pb
  • Biological Sciences::Biology::Ecology::Ecotoxicology
  • Q Science::QH Natural history. Biology::QH540 Ecology
Divisions
  • University of Nottingham, UK Campus::Faculty of Medicine and Health Sciences::School of Veterinary Medicine & Science
  • University of Nottingham, UK Campus::Faculty of Social Sciences::School of Geography
Deposit date
2022-06-13
Data type
Raw Field Data
Contributors
  • Johnson, Matthew
Funders
  • Natural Environment Research Council
  • Natural Resources Wales
Grant number
  • NE/L002604/1
Collection dates
  • September 2020 to May 2021
Coverage
  • Wales
Data collection method
Aquatic invertebrate communities were sampled following the Environmental Agency of England standard protocol (Her Majesty’s Stationary Office, 1985; Environment Agency, 2009) of a three-minute kick sample plus a one-minute hand search, using a 1 mm2 mesh net with an opening of 0.25 m wide and 0.22 m deep. The operator moved systematically upstream, ensuring, to the best of their ability, that different habitats were proportionally sampled through time based on spatial coverage. The collected sample was stored in alcohol to preserve the invertebrates. The invertebrates were sorted in the laboratory, with each individual identified to genus level, where possible, and counted. Those taxa that were not identified to genus level were identified instead to Family level, such as Chironomidae and Simuliidae, with the exception of Oligochaeta, which were identified to sub-class level. A consistent taxonomic resolution was used across all samples for comparative purposes. The invertebrates were then freeze dried to a constant mass and prepared for acid digestion. Any invertebrate weighing over 0.01 g (dry weight) was acid digested individually. For invertebrates weighing under 0.01 g, multiple individuals of the same family and collected at the same site and time were pooled together to reach a cumulative weight over 0.01 g. Individuals were grouped at the family level to allow for the pooling of a larger number of individuals. If a cumulative weight of over 0.01 g could not be reached, the invertebrates were not analysed. Invertebrate samples weighing over 0.01 g were acid digested with 4 mL 70% HNO3 and 1 mL H2O2, using a teflon-coated graphite hotplate block digester. The samples were then made up to 50 ml with MilliQ water, and the resultant solutions were diluted 1-in-10 with MilliQ water prior to elemental analysis by ICP-MS.
Resource languages
  • en
Publisher
The University of Nottingham
CC-BY
Except where otherwise noted, this item's license is described as Creative Commons by Attribution