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Discovery of a novel polymer for xeno-free, long-term culture of human pluripotent stem cell expansion

dc.contributor.authorNasir, Aishah
dc.contributor.authorThorpe, Jordan
dc.contributor.authorBurroughs, Laurence
dc.contributor.authorAlexander, Morgan
dc.contributor.authorDenning, Chris
dc.contributor.authorMeurs, Joris
dc.date.accessioned2021-01-20T13:44:38Z
dc.date.available2021-01-20T13:44:38Z
dc.date.issued2021-01-20
dc.identifier.urihttps://rdmc.nottingham.ac.uk/handle/internal/9118
dc.description.abstractDiscovery of poly(TCDMDA-blend-BA) polymer substrate offers a cost-effective and xeno-free adherent culture system for human pluripotent stem cells (hPSCs) in E8 medium. This is a scalable substrate which can be readily applied to common tissue culture plasticware (TCP), whilst retaining stem cell integrity after long-term serial passage. Fulfillment of all regulatory cell culture requirements makes this a desirable expansion platform for clinical-scale, therapeutic and biomedical applications. Data presented herein includes fluorescence microscopy of high-throughput attachment screening studies.en_UK
dc.language.isoen_USen_UK
dc.publisherThe University of Nottinghamen_UK
dc.rightsCC-BY*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.sourcehttps://onlinelibrary.wiley.com/doi/full/10.1002/adma.201501351en_UK
dc.subject.lcshStem cellsen_UK
dc.subject.lcshCell cultureen_UK
dc.subject.lcshBiomedical materialsen_UK
dc.titleDiscovery of a novel polymer for xeno-free, long-term culture of human pluripotent stem cell expansionen_UK
dc.identifier.doihttp://doi.org/10.17639/nott.7093
dc.subject.freePolymer microarray, Biomaterials discovery, Essential 8 medium, Xeno-free, human pluripotent stem cellsen_UK
dc.subject.jacsEngineering::General engineering::Bioengineering, biomedical engineering & clinical engineering::Biomaterialsen_UK
dc.subject.lcQ Science::QH Natural history. Biology::QH573 Cytologyen_UK
dc.date.collection01/12/15 - 01/08/19en_UK
uon.divisionUniversity of Nottingham, UK Campus::Faculty of Medicine and Health Sciences::School of Medicineen_UK
uon.funder.controlledEngineering & Physical Sciences Research Councilen_UK
uon.funder.controlledWellcome Trusten_UK
uon.datatypecell microscopy, Phosphokinase array, protein adsorption surface analysis, growth curves cell analysis, integrin blocking assaysen_UK
uon.funder.freeBritish Heart Foundationen_UK
uon.funder.freeNational Centre for the Replacement, Refinement, and Reduction of Animals in Research (NC3Rs)en_UK
uon.grantEP/N006615/1en_UK
uon.parentproject'Future-Proof' Synthetic Surfaces for the Automated Manufacture of Human Pluripotent Stem Cells (EP/H045384/1)en_UK
uon.collectionmethodCell microscopy (IMSTAR), Operetta (Perkin Elmer), Phosphokinase R&D kit, liquid extraction surface analysis-tandem mass spectrometry (LESA-MS/MS; TriVersa Nanomate- Advion Biosciences), Cell analysis with Cedex HiRes Analyzer-the Roche Cell Counteren_UK
uon.legalA patent has been filed in relation to the work described herein.en_UK
uon.rightscontactUniversity of Nottinghamen_UK
uon.institutes-centresUniversity of Nottingham, UK Campus::Biomolecular Sciences, Centre foren_UK
uon.preservation.rarelyaccessedtrue
uon.identifier.risproject5184113en_UK
dc.relation.doi10.1002/adhm.202001448en_UK


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