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dc.contributor.authorKotowska, Anna M
dc.contributor.authorScurr, David J
dc.contributor.otherTrindade, Gustavo F
dc.contributor.otherMendes, Paula M
dc.contributor.otherWilliams, Philip M
dc.contributor.otherAylott, Jonathan W
dc.contributor.otherShard, Alexander G
dc.contributor.otherAlexander, Morgan R
dc.date.accessioned2020-08-28T07:42:18Z
dc.date.available2020-08-28T07:42:18Z
dc.date.issued2020-08-28
dc.identifier.urihttps://rdmc.nottingham.ac.uk/handle/internal/8604
dc.description.abstractLabel-free protein characterisation at surfaces requires digestion or matrix application prior to mass spectrometry. We report the assignment of undigested proteins at surfaces using secondary ion mass spectrometry (SIMS). Ballistic fragmentation induced by a gas cluster ion beam (GCIB) produces peptide fragments for subsequent OrbitrapTM analysis. We annotate 16 example proteins (up to 272 kDa) by de novo sequencing and apply the methodology to characterising a protein monolayer biochip and in situ depth profiling of proteins through human skin.en_UK
dc.language.isoenen_UK
dc.publisherThe University of Nottinghamen_UK
dc.rightsCC-BY*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.lcshProteinsen_UK
dc.subject.lcshProteomicsen_UK
dc.subject.lcshSecondary ion mass spectrometryen_UK
dc.titleIn situ protein identification, mapping and depth profiling using 3D OrbiSIMSen_UK
dc.identifier.doihttp://doi.org/10.17639/nott.7070
dc.subject.freeOrbiSIMS; SIMS, proteins, proteomics, imaging, label-freeen_UK
dc.subject.jacsBiological Sciences::Molecular biology, biophysics & biochemistryen_UK
dc.subject.lcQ Science::QP Physiology::QP501 Animal biochemistryen_UK
uon.divisionUniversity of Nottingham, UK Campusen_UK
uon.funder.controlledEngineering & Physical Sciences Research Councilen_UK
uon.datatype3D OrbiSIMS spectra, images, depth profilesen_UK
uon.grantEP/P029868/1en_UK
uon.grantEP/L01646X/1en_UK
uon.grantEP/P031684/1en_UK
uon.collectionmethod3D OrbiSIMS, individual settings for different samples described in the methods sectionen_UK
uon.institutes-centresUniversity of Nottingham, UK Campusen_UK
uon.preservation.rarelyaccessedtrue


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