In situ protein identification, mapping and depth profiling using 3D OrbiSIMS
dc.contributor.author | Kotowska, Anna M | |
dc.contributor.author | Scurr, David J | |
dc.contributor.other | Trindade, Gustavo F | |
dc.contributor.other | Mendes, Paula M | |
dc.contributor.other | Williams, Philip M | |
dc.contributor.other | Aylott, Jonathan W | |
dc.contributor.other | Shard, Alexander G | |
dc.contributor.other | Alexander, Morgan R | |
dc.date.accessioned | 2020-08-28T07:42:18Z | |
dc.date.available | 2020-08-28T07:42:18Z | |
dc.date.issued | 2020-08-28 | |
dc.identifier.uri | https://rdmc.nottingham.ac.uk/handle/internal/8604 | |
dc.description.abstract | Label-free protein characterisation at surfaces requires digestion or matrix application prior to mass spectrometry. We report the assignment of undigested proteins at surfaces using secondary ion mass spectrometry (SIMS). Ballistic fragmentation induced by a gas cluster ion beam (GCIB) produces peptide fragments for subsequent OrbitrapTM analysis. We annotate 16 example proteins (up to 272 kDa) by de novo sequencing and apply the methodology to characterising a protein monolayer biochip and in situ depth profiling of proteins through human skin. | en_UK |
dc.language.iso | en | en_UK |
dc.publisher | The University of Nottingham | en_UK |
dc.rights | CC-BY | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.lcsh | Proteins | en_UK |
dc.subject.lcsh | Proteomics | en_UK |
dc.subject.lcsh | Secondary ion mass spectrometry | en_UK |
dc.title | In situ protein identification, mapping and depth profiling using 3D OrbiSIMS | en_UK |
dc.identifier.doi | http://doi.org/10.17639/nott.7070 | |
dc.subject.free | OrbiSIMS; SIMS, proteins, proteomics, imaging, label-free | en_UK |
dc.subject.jacs | Biological Sciences::Molecular biology, biophysics & biochemistry | en_UK |
dc.subject.lc | Q Science::QP Physiology::QP501 Animal biochemistry | en_UK |
uon.division | University of Nottingham, UK Campus | en_UK |
uon.funder.controlled | Engineering & Physical Sciences Research Council | en_UK |
uon.datatype | 3D OrbiSIMS spectra, images, depth profiles | en_UK |
uon.grant | EP/P029868/1 | en_UK |
uon.grant | EP/L01646X/1 | en_UK |
uon.grant | EP/P031684/1 | en_UK |
uon.collectionmethod | 3D OrbiSIMS, individual settings for different samples described in the methods section | en_UK |
uon.institutes-centres | University of Nottingham, UK Campus | en_UK |
uon.preservation.rarelyaccessed | true |
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