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dc.contributor.authorMeurs, Joris
dc.contributor.authorAlexander, Morgan
dc.contributor.otherMeurs, Joris
dc.contributor.otherAlexander, Morgan
dc.contributor.otherWidmaier, Simon
dc.contributor.otherLevkin, Pavel
dc.contributor.otherBarrett, David
dc.contributor.otherKim, Dong-Hyun
dc.date.accessioned2018-12-21T11:02:38Z
dc.date.available2018-12-21T11:02:38Z
dc.date.issued2018-12-21
dc.identifier.urihttps://rdmc.nottingham.ac.uk/handle/internal/6183
dc.identifier.urihttp://dx.doi.org/10.17639/nott.6176
dc.description.abstractData related to the paper "Improved Extraction Repeatability and Spectral Reproducibility for Liquid Extraction Surface Analysis–Mass Spectrometry Using Superhydrophobic–Superhydrophilic Patterning" A major problem limiting reproducible use of liquid extraction surface analysis (LESA) array sampling of dried surface-deposited liquid samples is the unwanted spread of extraction solvent beyond the dried sample limits, resulting in unreliable data. Here, we explore the use of the Droplet Microarray (DMA), which consists of an array of superhydrophilic spots bordered by a superhydrophobic material giving the potential to confine both the sample spot and the LESA extraction solvent in a defined area. We investigated the DMA method in comparison with a standard glass substrate using LESA analysis of a mixture of biologically relevant compounds with a wide mass range and different physicochemical properties. The optimized DMA method was subsequently applied to urine samples from a human intervention study. Relative standard deviations for the signal intensities were all reduced at least 3-fold when performing LESA-MS on the DMA surface compared with a standard glass surface. Principal component analysis revealed more tight clusters indicating improved spectral reproducibility for a human urine sample extracted from the DMA compared to glass. Lastly, in urine samples from an intervention study, more significant ions (145) were identified when using LESA-MS spectra of control and test urine extracted from the DMA. We demonstrate that DMA provides a surface-assisted LESA-MS method delivering significant improvement of the surface extraction repeatability leading to the acquisition of more robust and higher quality data. The DMA shows potential to be used for LESA-MS for controlled and reproducible surface extraction and for acquisition of high quality, qualitative data in a high-throughput manner.en_UK
dc.language.isoenen_UK
dc.publisherThe University of Nottinghamen_UK
dc.relation.urihttp://doi.org/10.1021/acs.analchem.8b00973en_UK
dc.subject.lcshSurfaces (Technology) -- Analysisen_UK
dc.subject.lcshMass spectrometryen_UK
dc.subject.lcshSurface chemistry -- Techniqueen_UK
dc.titleImproved extraction repeatability and spectral reproducibility for liquid extraction surface analysis–mass spectrometry using superhydrophobic–superhydrophilic patterningen_UK
dc.title.alternativeLESA-MS data of a standard mixture sampled from glass and Droplet Microarrayen_UK
dc.title.alternativeLESA-MS data of urine samples from a tea intervention study sampled from glass and Droplet Microarrayen_UK
dc.subject.freeLESA-MS, Droplet Microarray, Sampling Repeatabilityen_UK
dc.subject.jacsBiological Sciences::Molecular biology, biophysics & biochemistry::Applied molecular biology, biophysics & biochemistryen_UK
dc.subject.lcQ Science::QP Physiology::QP501 Animal biochemistryen_UK
dc.contributor.corporateKarlsruhe Institute of Technologyen_UK
uon.divisionUniversity of Nottingham, UK Campus::Faculty of Science::School of Pharmacyen_UK
uon.funder.controlledEngineering & Physical Sciences Research Councilen_UK
uon.datatypeLESA-MS spectraen_UK
uon.grantEP/N006615/1en_UK
uon.collectionmethodDried samples were extracted from the surface using the Nanomate robot (Advion Biosciences, Ithaca, USA) using a solvent mixture containing 70% methanol, 30% water after which formic acid was added to 0.1% v/v. The extraction solvent (5 μL) was aspirated from the solvent reservoir from which 3 μL was dispensed onto the substrate surface. After 5 s, 3.5 μL was re-aspirated into the tip and, after a delay of 10 s, the tip was directed to a nanoESI chip. Ionization was performed at 1.45 kV with 0.3 psi back pressure.3 Data was acquired for 1.5 min in electrospray positive ionization mode on an Exactive Orbitrap mass spectrometer (Thermo Scientific, San Jose, USA) using a scan range of m/z 100−1500. The resolution was set to 100 000 at m/z 400. Maximum injection was set to 1000 ms; the AGC target was set to 1 × 106 , and every scan consisted of 1 microscan. The capillary temperature was set to 250 °C, and the declustering potential, skimmer voltage, and capillary voltage were, respectively, set to 125, 20, and 80 Ven_UK
uon.institutes-centresUniversity of Nottingham, UK Campusen_UK
uon.preservation.rarelyaccessedtrue


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