Raw data and analysis for paper "Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion"

Hammad, Moamen; Smith, James; Rao, Wei

dc.contributor.author	Hammad, Moamen
dc.contributor.author	Smith, James
dc.contributor.author	Rao, Wei
dc.date.accessioned	2016-07-22T14:35:29Z
dc.date.available	2016-07-22T14:35:29Z
dc.date.issued	2016-07-21
dc.identifier.uri	https://rdmc.nottingham.ac.uk/handle/internal/52
dc.description.abstract	The two aims 1) identification of protein uniquely adsorbed to plasma etched tissue culture polystyrene using proteomics; 2) quantify and analyse pluripotent cell adherence to a range of arrayed micro environments on N-(4-hydroxyphenyl)methacryalamide. Purpose to identify novel protein pretreatments using high throughput screening. Previous publications have shown human pluripotent adherence to the plasma etched tissue culture polystyrene and N-(4-hydroxyphenyl)methacryalamide.	en_UK
dc.language.iso	en	en_UK
dc.publisher	University of Nottingham	en_UK
dc.subject.lcsh	Protein engineering	en_UK
dc.subject.lcsh	Proteomics	en_UK
dc.subject.lcsh	Cell adhesion	en_UK
dc.subject.lcsh	Tissue culture	en_UK
dc.title	Raw data and analysis for paper "Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion"	en_UK
dc.identifier.doi	http://doi.org/10.17639/nott.49
dc.subject.free	Protein adsorption; proteomics; pluripotent cell adherence; protein pretreatments; N-(4-hydroxyphenyl)methacryalamide; plasma etched tissue culture polystyrene; high throughput screening; heat shock proteins	en_UK
dc.subject.jacs	Biological Sciences::Molecular biology, biophysics & biochemistry::Biomolecular science	en_UK
dc.subject.lc	Q Science::QH Natural history. Biology	en_UK
dc.subject.lc	Q Science::QR Microbiology	en_UK
dc.date.collection	May 2014-July 2014	en_UK
uon.division	University of Nottingham, UK Campus::Faculty of Science::School of Pharmacy	en_UK
uon.funder.controlled	Engineering & Physical Sciences Research Council	en_UK
uon.datatype	Word files; Tif files; Excel files	en_UK
uon.grant	EP/H045384/1	en_UK
uon.collectionmethod	An Ix51 IMSTAR microscope was used for all image acquisition. The IMSTAR microscope is equipped with an automated stage making it suitable for investigating large numbers of arrayed materials on a single slide. Acquisition was achieved using the in-built software; by generating a position list to image each arrayed spot or imaging an operator defined area. For the primary screening array a position list of 36 x 92 positions was generated and captured. For the secondary screening array a position list of 35 x 105 positions was generated and captured. Each position represents one polymer spot. Focus was calculated based on operator assigned landmarks on the area extremities. All images were captured using a ProgRes MF (Jenoptik) monochrome CCD digital camera.	en_UK