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Raw data and analysis for paper "Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion"
|dc.description.abstract||The two aims 1) identification of protein uniquely adsorbed to plasma etched tissue culture polystyrene using proteomics; 2) quantify and analyse pluripotent cell adherence to a range of arrayed micro environments on N-(4-hydroxyphenyl)methacryalamide. Purpose to identify novel protein pretreatments using high throughput screening. Previous publications have shown human pluripotent adherence to the plasma etched tissue culture polystyrene and N-(4-hydroxyphenyl)methacryalamide.||en_UK|
|dc.publisher||University of Nottingham||en_UK|
|dc.title||Raw data and analysis for paper "Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion"||en_UK|
|dc.subject.free||Protein adsorption; proteomics; pluripotent cell adherence; protein pretreatments; N-(4-hydroxyphenyl)methacryalamide; plasma etched tissue culture polystyrene; high throughput screening; heat shock proteins||en_UK|
|dc.subject.jacs||Biological Sciences::Molecular biology, biophysics & biochemistry::Biomolecular science||en_UK|
|dc.subject.lc||Q Science::QH Natural history. Biology||en_UK|
|dc.subject.lc||Q Science::QR Microbiology||en_UK|
|dc.date.collection||May 2014-July 2014||en_UK|
|uon.division||University of Nottingham, UK Campus::Faculty of Science::School of Pharmacy||en_UK|
|uon.funder.controlled||Engineering & Physical Sciences Research Council||en_UK|
|uon.datatype||Word files; Tif files; Excel files||en_UK|
|uon.collectionmethod||An Ix51 IMSTAR microscope was used for all image acquisition. The IMSTAR microscope is equipped with an automated stage making it suitable for investigating large numbers of arrayed materials on a single slide. Acquisition was achieved using the in-built software; by generating a position list to image each arrayed spot or imaging an operator defined area. For the primary screening array a position list of 36 x 92 positions was generated and captured. For the secondary screening array a position list of 35 x 105 positions was generated and captured. Each position represents one polymer spot. Focus was calculated based on operator assigned landmarks on the area extremities. All images were captured using a ProgRes MF (Jenoptik) monochrome CCD digital camera.||en_UK|