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dc.contributor.authorHammad, Moamen
dc.contributor.authorSmith, James
dc.contributor.authorRao, Wei
dc.date.accessioned2016-07-22T14:35:29Z
dc.date.available2016-07-22T14:35:29Z
dc.date.issued2016-07-21
dc.identifier.urihttps://rdmc.nottingham.ac.uk/handle/internal/52
dc.description.abstractThe two aims 1) identification of protein uniquely adsorbed to plasma etched tissue culture polystyrene using proteomics; 2) quantify and analyse pluripotent cell adherence to a range of arrayed micro environments on N-(4-hydroxyphenyl)methacryalamide. Purpose to identify novel protein pretreatments using high throughput screening. Previous publications have shown human pluripotent adherence to the plasma etched tissue culture polystyrene and N-(4-hydroxyphenyl)methacryalamide.en_UK
dc.language.isoenen_UK
dc.publisherUniversity of Nottinghamen_UK
dc.subject.lcshProtein engineeringen_UK
dc.subject.lcshProteomicsen_UK
dc.subject.lcshCell adhesionen_UK
dc.subject.lcshTissue cultureen_UK
dc.titleRaw data and analysis for paper "Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion"en_UK
dc.identifier.doihttp://doi.org/10.17639/nott.49
dc.subject.freeProtein adsorption; proteomics; pluripotent cell adherence; protein pretreatments; N-(4-hydroxyphenyl)methacryalamide; plasma etched tissue culture polystyrene; high throughput screening; heat shock proteinsen_UK
dc.subject.jacsBiological Sciences::Molecular biology, biophysics & biochemistry::Biomolecular scienceen_UK
dc.subject.lcQ Science::QH Natural history. Biologyen_UK
dc.subject.lcQ Science::QR Microbiologyen_UK
dc.date.collectionMay 2014-July 2014en_UK
uon.divisionUniversity of Nottingham, UK Campus::Faculty of Science::School of Pharmacyen_UK
uon.funder.controlledEngineering & Physical Sciences Research Councilen_UK
uon.datatypeWord files; Tif files; Excel filesen_UK
uon.grantEP/H045384/1en_UK
uon.collectionmethodAn Ix51 IMSTAR microscope was used for all image acquisition. The IMSTAR microscope is equipped with an automated stage making it suitable for investigating large numbers of arrayed materials on a single slide. Acquisition was achieved using the in-built software; by generating a position list to image each arrayed spot or imaging an operator defined area. For the primary screening array a position list of 36 x 92 positions was generated and captured. For the secondary screening array a position list of 35 x 105 positions was generated and captured. Each position represents one polymer spot. Focus was calculated based on operator assigned landmarks on the area extremities. All images were captured using a ProgRes MF (Jenoptik) monochrome CCD digital camera.en_UK


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