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dc.contributor.authorStarr, Nichola
dc.contributor.authorJohnson, Daniel
dc.contributor.authorScurr, David
dc.description.abstractThis work demonstrates the ability to detect changes in both quantity and spatial distribution of human stratum corneum (SC) lipids from samples collected in vivo. The SC functions as the predominant barrier to the body, protecting against the penetration of xenobiotic substances. Changes to the SC lipid composition have been associated with barrier impairment and consequent skin disorders and it is therefore important to monitor and quantify changes to this structure. This work demonstrates the first reported use of time of flight secondary ion mass spectrometry (ToF-SIMS) to assess physiological changes to human SC as a function of depth. This technique provides exceptional sensitivity and chemical specificity, allowing analysis of single tape stripped samples taken from volunteers. Using this methodology we were able to successfully identify chemical differences in human SC resulting from both intrinsic and extrinsic (photo) aging. Samples were collected from women of two age groups (under 27 and post-menopausal) and from two body sites with varying UV exposure (inner forearm and dorsal hand) and differences were identified using multivariate data analysis. The key finding was the significant aged-related increase and change in spatial distribution of the sterol cholesterol sulfate, a membrane stabilizing lipid. Significant changes in the prevalence of both lignoceric acid (C24:0) and hexacosanoic acid (C26:0) were also observed. This work describes previously unreported age-related chemical changes to human SC, providing an insight into aging mechanisms which may improve the design of both pharmaceutical and cosmetic topical products.en_UK
dc.publisherUniversity of Nottinghamen_UK
dc.subject.lcshTime-of-flight mass spectrometryen_UK
dc.subject.lcshSkin –- Agingen_UK
dc.subject.lcshSkin -- Physiologyen_UK
dc.subject.lcshOintments -- Designen_UK
dc.titleAge-related changes to human stratum corneum lipids detected using ToF-SIMS following in vivo samplingen_UK
dc.subject.freeStratum corneum lipids; skin aging; photo-aging; human stratum corneum; in vivo sampling; tape stripping; cholesterol sulfate; time of flight secondary ion mass spectroscopy, ToF-SIMSen_UK
dc.subject.jacsJACS Subjects::Subjects Allied to Medicine::Anatomy, physiology & pathologyen_UK
dc.subject.lcLibrary of Congress Subject Areas::Q Science::QM Human anatomyen_UK 2013-May 2015en_UK
uon.divisionFaculties, Schools and Departments::University of Nottingham, UK Campus::Faculty of Science::School of Pharmacyen_UK
uon.funder.controlledFunders::Engineering & Physical Sciences Research Councilen_UK
uon.datatypeAFM measurements (Excel file); SEM images; ToF-SIMS spectra exported as both text and Excel files; ToF-SIMS secondary ion images (exported as PDF files)en_UK
uon.collectionmethodAtomic force microscopy (AFM); Scanning electron microscopy (SEM); Time of flight secondary ion mass spectrometry (ToF-SIMS)en_UK
uon.rightscontactUniversity of Nottinghamen_UK

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