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dc.contributor.authorHüsler, Amanda
dc.contributor.authorAlexander, Morgan
dc.contributor.otherHaas, Simon
dc.contributor.otherParry, Luke
dc.contributor.otherRomero, Manuel
dc.contributor.otherWilliams, Paul
dc.contributor.otherWildman, Ricky
dc.description.abstractData relating to the paper: "Effect of surfactant on Pseudomonas aeruginosa colonization of polymer microparticles and flat films." Micro- and nanoparticles are of great interest because of their potential for trafficking into the body for applications such as low-fouling coatings on medical devices, drug delivery in pharmaceutics and cell carriers in regenerative medicine strategies. Particle production often relies on the use of surfactants to promote stable droplet formation. However, the presence of residual surfactant has been shown to complicate the surface chemistry and resultant properties. When forming particles from polymerizable monomer droplets, these polymeric surfactant chains can become physically entangled in the particle surface. Due to the key role of the outermost layers of the surface in biomaterial interactions, the surface chemistry and its influence on cells needs to be characterized. This is the first study to assess surfactant retention on microfluidic produced particles and its effect on bacterial attachment; surfactant contaminated microparticles are compared with flat films which are surfactant-free. Polymeric microparticles with an average diameter of 76 ± 1.7 μm were produced by using a T-junction microfluidic system to form monomer droplets which were subsequently photopolymerized. Acrylate based monomer solutions were found to require 2 wt% PVA to stabilize droplet formation. ToF-SIMS was employed to assess the surface chemistry revealing the presence of PVA in a discontinuous layer on the surface of microparticles which was reduced but not removed by solvent washing. The effect of PVA on bacterial (Pseudomonas aeruginosa) attachment was quantified and showed reduction as a function of the amount of PVA retained at the surface. The insights gained in this study help define the structure–function relationships of the particulate biomaterial architecture, supporting materials design with biofilm control.en_UK
dc.publisherThe University of Nottinghamen_UK
dc.sourceDOI: 10.1038/nbt.2316 ; DOI: 10.1002/adma.201204936en_UK
dc.subject.lcshBiomedical materialsen_UK
dc.subject.lcshSurface active agentsen_UK
dc.subject.lcshPseudomonas aeruginosaen_UK
dc.titleEffect of surfactant on Pseudomonas aeruginosa colonization of polymer microparticles and flat filmsen_UK
dc.subject.freeMicroparticles, Microfluidics, Biomaterials, Photopolymerisation, Surfactant, Pseudomonas aeruginosaen_UK
dc.subject.jacsSubjects Allied to Medicine::Pharmacology, toxicology & pharmacy::Pharmacologyen_UK
dc.subject.lcT Technology::TA Engineering (General). Civil engineering (General)en_UK
dc.subject.lcQ Science::QD Chemistry::QD450 Physical and theoretical chemistryen_UK
dc.subject.lcR Medicine::RS Pharmacy and materia medicaen_UK – 01/2017en_UK
uon.divisionUniversity of Nottingham, UK Campus::Faculty of Science::School of Pharmacyen_UK
uon.divisionUniversity of Nottingham, UK Campus::Faculty of Engineeringen_UK
uon.funder.controlledEngineering & Physical Sciences Research Councilen_UK
uon.datatypeToF-SIMS data, Confocal microscope data, SEM images, Videosen_UK
uon.collectionmethod- IONTOF IV instrument (ION-TOF GmbH): 25 kV Bi3+ high current bunched mode, pulsed target current of ~1 pA, 500 × 500 µm, 256 × 256 pixels, ion dose of 2.45 × 1011 ions per cm2, mass resolution of >7000, charge compensation low energy (20 eV) electron; - ZEISS LSM 700 laser confocal microscope: 639 × 639 µm, 512 × 512 pixels, 8-bit color depth, excitation at 555 nm, power of 10%, 10x/0.30 M27 EC Plan-Neofluar objective, 1 Airy unit (AU); - Confocal image processing: in-built MATLAB (R2016b, version shape recognising function combined with adapted scripts from the COMSTAT program; - JEOL JSM-6060LV scanning electron microscope: 10 – 20 kV; - Videos: Ti-S inverted fluorescence microscope (Nikon UK Ltd, UK), magnification of 20x, NA of 0.45, working distance of WD = 6.9-8.2 mm, UX100 Mini high-speed camera (Photron Ltd, UK), frame rate of 10,000– 20,000 frames per second, 1240 × 480 pixels, pixel size of 10 µm.en_UK
uon.rightscontactThe University of Nottinghamen_UK

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