An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 6147R-1

Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’.

RNAi line: 6147R-1 (III)
Source: NIG

Name: TSC1 (2)
Full name: Tsc1
Also known as: dTsc1, TSC, rocky
Annotation symbol: CG6147
FlyBase ID: FBgn0026317

File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)

Included files: 040913_An9_Lgl_6147R1_w1_combined.tif 040913_An9_Lgl_6147R1_w2_combined.tif 110913_An1_Lgl_6147R1_w1_combined.tif 110913_An1_Lgl_6147R1_w2_combined.tif 180913_An12_Lgl_6147R1_combined.tif 180913_An13_Lgl_6147R1_w1_combined.tif 180913_An13_Lgl_6147R1_w2_combined.tif 190913_6147R1_A4_w1_combined.tif 190913_6147R1_A4_w2_combined.tif 290813_An4_Lgl_6147R1_w_combined.tif