An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 14026R-3

Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’.

RNAi line: 14026R-3 (III)
Source: NIG

Name: tkv (2)
Full name: thickveins
Also known as: str, Brk25D, dtfr, l(2)04415
Annotation symbol: CG14026
FlyBase ID: FBgn0003716

File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)

Included files: 030913_Lgl14026R3An3w1_combined.tif 030913_Lgl14026R3An5w1_combined.tif 190713_Lgl14026R3An7w1_combined.tif 290813_An5_Lgl_14026R3_w_combined.tif 300813_Lgl14026R3An2w1_combined.tif 300813_Lgl14026R3An4w1_combined.tif 300813_Lgl14026R3An4w2_combined.tif