An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 14026R-1

Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’.

RNAi line: 14026R-1 (III)
Source: NIG

Name: tkv (1)
Full name: thickveins
Also known as: str, Brk25D, dtfr, l(2)04415
Annotation symbol: CG14026
FlyBase ID: FBgn0003716

File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)

Included files: 030913_Lgl14026R1An4w1_combined.tif 030913_Lgl14026R1An4w2_combined.tif 040913_An10_Lgl_14026R1_w1_combined.tif 040913_An10_Lgl_14026R1_w2_combined.tif 050913_14026R1_A3_W1_combined.tif 050913_14026R1_A5_W1_combined.tif 160713_Lgl14026R1An1w1_combined.tif 180215_Lgl14026R1An3_combined.tif 190215_An5_14026r1_w_combined.tif 200613_lgl_14026r1_an2__combined.tif 241014_lgl_14026R1_An1_combined.tif 250613_Lgl14026R1An6w1_combined.tif 250713_An1_Lgl_14026R1_w__combined.tif 250713b_An1_Lgl_14026R1_w__combined.tif 260613_An13_Lgl_14026R1_w_combined.tif 291014_Lgl14026R1An7w1_combined.tif 291014_Lgl14026R1An7w2_combined.tif 311014_Lgl14026R1_An5_combined.tif