An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 5671R-1

Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’.

RNAi line: 5671R-1 (III)
Source: NIG

Name: pTEN (2)
Full name: Phosphatase and tensin homolog
Also known as: dPTEN
Annotation symbol: CG5671
FlyBase ID: FBgn0026379

File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)

Included files: 020713_An2_Lgl_5671R1_w_combined.tif 020713_An3_Lgl_5671R1_w_combined.tif 040713_lgl_5671r1_a9_w1_combined.tif 100713_5671R1_A2_w1_combined.tif 100713_5671R1_A3_w1_combined.tif 100713_5671R1_A4_w1_combined.tif 100713_5671R1_A7_w1_combined.tif 160713_Lgl5671R1An2w1_combined.tif 260613_An7_Lgl_5671R1_w_combined.tif