An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 7467R-1

Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’.

RNAi line: 7467R-1 (III)
Source: NIG

Name: osa (1)
Full name: osa
Also known as: eld, C819, l(3)00090
Annotation symbol: CG7467
FlyBase ID: FBgn0261885

File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)

Included files: 011014_Lgl7467R1An6_combined.tif 111114_lgl_7467R1_An4_combined.tif 250914_7467r1_an4_w_combined.tif 250914_7467r1_an5_w_combined.tif 300914.lei - Lgl7462R1An4.tif