Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’.
RNAi line: LGL Alone
Source: Bloomington
Name: lgl mutant alone
Full name: lethal (2) giant larvae
Also known as: lgl, p127, dlgl, p127l(2)gl, l(2)gl
Annotation symbol: CG2671
FlyBase ID: FBgn0002121
File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)
Included files: 011112.lei - Lgl Animal2 whole1.tif 011112.lei - Lgl Animal2 whole2.tif 011112.lei - Lgl Animal3 whole1.tif 011112.lei - Lgl Animal3 whole2.tif 011112.lei - Lgl Animal 4 whole.tif 170413.lei - An 10 Lgl w1.tif 170413.lei - An4 Lgl w1.tif 170413.lei - An4 Lgl w2.tif 170413.lei - An8 lgl w1.tif 170413.lei - An8 Lgl w2.tif 170413.lei - An8 Lgl w3.tif 170413.lei - An9 Lgl w1.tif 170413.lei - An9 Lgl w2.tif 251012.lei - lgl Animal1 whole.tif 251012.lei - lgl Animal3 whole1.tif 251012.lei - lgl Animal 4 whole.tif