An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 40869

Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’.

RNAi line: 40869 (III)
Source: Bloomington

Name: crb (2)
Full name: crumbs
Also known as: Crumbs, l(3)07207, Crbs, far, l(3)j1B5
Annotation symbol: CG6383
FlyBase ID: FBgn0259685

File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)

Included files: 020216_An28_40869_w_combined.tif 040216_An3_40869_w_combined.tif 050216_An6_40869_w_combined.tif 101216_lgl40869An3_combined.tif An16 40869 w.tif