An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 8896R3

Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’.

RNAi line: 8896R3 (III)
Source: NIG

Name: 18w (1)
Full name: 18 wheeler
Also known as: Toll-2, tlr
Annotation symbol: CG8896
FlyBase ID: FBgn0004364

File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)

Included files: 18w An1_01122015.lei - An21 8896R-3 w.tif 18w_An2_01122015.lei - An22 8896R-3 w.tif 18w_An3A_02122015.lei - An12 8896R-3 w1.tif 18w_An3B_02122015.lei - An12 8896R-3 w2.tif 18w_An4_03122015.lei - An10 8896R-3 w.tif 18w_An6_04122015.lei - An7 8896R-3 w.tif