README: Nematode Surface Chemistry Dataset
Title: Nematode Surface Chemistry Dataset
Author: Dr. Veeren Chauhan
Publication Date: March 5, 2024
DOI: https://doi.org/10.17639/nott.7386

1. Description
This dataset contains surface chemistry profiles of wild-type Caenorhabditis elegans (N2) and Pristionchus pacificus (PS312), along with their respective daf-22 mutant strains (C. elegans DR476 and P. pacificus RS270/RS2204).

The dataset was generated using 3D-OrbiSIMS mass spectrometry with an argon gas cluster ion beam (Ar3000+, 20 keV) for surface sputtering and an Orbitrap analyzer for high-resolution detection (mass resolving power >240,000 at m/z 200).

Surface analysis was confined to the outermost ~50 nm, enabling detailed profiling of species-specific and developmentally regulated lipid compositions.

This dataset supports research in:
- Nematode physiology
- Lipid metabolism
- β-oxidation
- Interspecies interactions

2. Dataset Contents
The dataset is provided as a CSV (Comma-Separated Values) file with the following structure:

| Column Name          | Description |
|----------------------|-------------|
| Sample_ID           | Unique identifier for each sample |
| Species            | C. elegans (N2/DR476) or P. pacificus (PS312/RS270/RS2204) |
| Genotype           | Wild-type or daf-22 mutant |
| Developmental_Stage | Developmental stage of the nematode |
| Lipid_Compound      | Identified lipid species |
| m/z                | Mass-to-charge ratio of detected ions |
| Intensity          | Intensity of detected lipid signal |
| Replicate          | Biological replicate number |

3. Data Collection Methodology
- Instrument Used: 3D-OrbiSIMS
- Ion Source: Argon gas cluster ion beam (Ar3000+, 20 keV)
- Mass Analyzer: Orbitrap (>240,000 resolution at m/z 200)
- Depth Profiled: ~50 nm surface layer
- Number of Biological Replicates: 9 per condition

4. File Format
- Dataset File: Nematode_Surface_Chemistry_Dataset_Dr_Veeren_Chauhan_2024.csv
- Metadata & Documentation: README.txt (this file)

5. Usage & Citation
Researchers are encouraged to cite this dataset using the following format:

> Chauhan, V. (2024). Nematode Surface Chemistry Dataset. University of Nottingham. DOI: https://doi.org/10.17639/nott.7386

6. License & Permissions
- This dataset is made available under the CC BY 4.0 License.
- You are free to share, adapt, and use this dataset, provided that appropriate credit is given.

Contact Information:
Dr. Veeren Chauhan
School of Pharmacy
University of Nottingham
Boots Science Building, Nottingham, NG7 2RD
Email: veeren.chauhan@nottingham.ac.uk
Website: www.veerenchauhan.com